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Distal-less homeobox transcription factors regulate development and maturation of natural killer cells

Author:
Sunwoo, John B., Kim, Sungjin, Yang, Liping, Naik, Tina, Higuchi, Darryl A., Rubenstein, John L., Yokoyama, Wayne M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.31 pp. 10877-10882
ISSN:
0027-8424
Subject:
animal models, bone marrow, gene expression, genes, immatures, mice, natural killer cells, transcription (genetics), transcription factors
Abstract:
Natural killer (NK) cells constitute a subpopulation of lymphocytes that develop from precursors in the bone marrow (BM), but the transcriptional regulation of their development and maturation is only beginning to be understood, in part due to their relatively rare abundance, especially of developmental subsets. Using a mouse model in which NK cells are arrested at an immature stage of development, and a gene expression profiling approach, we uncovered transient normal NK cell expression of a homeobox transcription factor (TF) family, called Distal-less (Dlx), which had been primarily implicated in murine CNS, craniofacial, limb, and skin development. Our studies demonstrate that Dlx1, Dlx2, and Dlx3 are transiently expressed in immature Mac-1lo NK cells within the BM, with Dlx3 being the predominantly expressed member. These genes are expressed in a temporally regulated pattern with overlapping waves of expression, and they display functional redundancy. Expression is extinguished in fully mature splenic NK cells, and persistent expression of Dlx genes leads to functionally immature NK cells arrested at the Mac-1lo stage. Whereas conventional splenic NK cells develop but are arrested at an immature stage, there appears to be a complete failure to develop CD127⁺ thymic NK cells when Dlx genes are persistently expressed. We also observed that T and B cells fail to develop in the context of persistent Dlx1 expression. Thus, these studies indicate that Dlx TFs play a functional role in lymphocyte development.
Agid:
2359718