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TFIID component TAF7 functionally interacts with both TFIIH and P-TEFb

Author:
Gegonne, Anne, Weissman, Jocelyn D., Lu, Hanxin, Zhou, Meisheng, Dasgupta, Arindam, Ribble, Robert, Brady, John N., Singer, Dinah S.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.14 pp. 5367-5372
ISSN:
0027-8424
Subject:
models, protein-protein interactions, transcription (genetics), transcription factors
Abstract:
Transcription consists of a series of highly regulated steps: assembly of the preinitiation complex (PIC) at the promoter, initiation, elongation, and termination. PIC assembly is nucleated by TFIID, a complex composed of the TATA-binding protein (TBP) and a series of TBP-associated factors (TAFs). One component, TAF7, is incorporated in the PIC through its interaction with TFIID but is released from TFIID upon transcription initiation. We now report that TAF7 interacts with the transcription factors, TFIIH and P-TEFb, resulting in the inhibition of their Pol II CTD kinase activities. Importantly, in in vitro transcription reactions, TAF7 inhibits steps after PIC assembly and formation of the first phosphodiester bonds. Further, in vivo TAF7 coelongates with P-TEFb and Pol II downstream of the promoter. We propose a model in which TAF7 contributes to the regulation of the transition from PIC assembly to initiation and elongation.
Agid:
2361100