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Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

Author:
Sun, Ning, Panetta, Nicholas J., Gupta, Deepak M., Wilson, Kitchener D., Lee, Andrew, Jia, Fangjun, Hu, Shijun, Cherry, Athena M., Robbins, Robert C., Longaker, Michael T., Wu, Joseph C.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.37 pp. 15720-15725
ISSN:
0027-8424
Subject:
adults, biopsy, fibroblasts, humans, induced pluripotent stem cells, patients, somatic cells, transcription factors
Abstract:
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.
Agid:
2366036