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Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b

Author:
Kreft, Jürgen, Funke, Dorothee, Haas, Albert, Lottspeich, Friedrich, Goebel, Werner
Source:
FEMS microbiology letters 1989 v.57 no.2 pp. 197-202
ISSN:
0378-1097
Subject:
Listeria ivanovii, Listeria monocytogenes, amino acid sequences, enzyme activity, hemolysins, hemolysis, molecular weight
Abstract:
In culture supernatants of both Listeria ivanovii and Listeria monocytogenes Sv4b, for the first time a hemolysin of molecular weight 58 kDa was identified, which had all the characteristics of an SH-activated cytolysin, and which was therefore identified as listeriolysin O (LLO). In the case of L. ivanovii a second major supernatant protein of molecular weight 24 kDa co-purified with LLO. However, the function of this protein has to be determined. In culture supernatants of L. ivanovii a sphingomyelinase and a lecithinase activity could be detected, both enzymatic activities together contributing to the pronounced hemolysis caused by L. ivanovii. The N-terminal amino acid sequences of LLO and the 24 kDa from L. ivanovii are shown.
Agid:
2373865