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Short-term storage of alginate-encapsulated protocorm-like bodies of phalaenopsis cornu-cervi (breda) blume & Rchb. F.

Author:
Rittirat, S., Klaocheed, S., Thammasiri, K.
Source:
Acta horticulturae 2015 no.1078 pp. 75-80
ISSN:
0567-7572
Subject:
Phalaenopsis, activated carbon, calcium chloride, encapsulation, flow cytometry, germination, naphthaleneacetic acid, plantlets, ploidy, sodium alginate, sucrose, survival rate, temperature
Abstract:
Protocorm-like bodies (PLB) of Phalaenopsis cornu-cervi (Breda) Blume & Rchb. f. derived from wounded protocorm segments culturing on ½ Murashige and Skoog, 1962 (MS) medium containing 0.1 mg/L NAA and 0.1 mg/L TDZ. Encapsulation of the PLB using 3% sodium alginate and 100 mM calcium chloride dissolved in ND liquid medium supplemented 2% (w/v) sucrose. Among the three temperature regimes of storage (4±1°C, 8±1°C, 25±1°C), encapsulated PLB stored at 25±1°C, for 30 days showed maximum percentage of germination at 100 when culture on ND medium supplemented with 4% (w/v) sucrose with 0.2% (w/v) activated charcoal (AC). Plantlets regenerated from capsules were acclimatized successfully with 100% survival rate. Morphological characteristics of those plantlets were normal and ploidy level was the same as controlled plantlets as revealed by flow cytometry.
Agid:
2386925