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Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose-fermenting yeast Pachysolen tannophilus

Author:
Bolen, P.L., Roth, K.A., Freer, S.N.
Source:
Applied and environmental microbiology 1986 v.52 no.4 pp. 660
ISSN:
0099-2240
Subject:
lignocellulose, yeasts, xylose, fermentation, aldoses, xylitol, oxidoreductases, biochemistry, enzyme activity, purification, ethanol
Abstract:
Although xylose is a major product of hydrolysis of lignocellulosic materials, few yeasts are able to convert it to ethanol. In Pachysolen tannophilus, one of the few xylose-fermenting yeasts found, aldose reductase and xylitol dehydrogenase were found to be key enzymes in the metabolic pathway for xylose fermentation. This paper presents a method for the rapid and simultaneous purification of both aldose reductase and xylitol dehydrogenase from P. tannophilus. Preliminary studies indicate that this method may be easily adapted to purify similar enzymes from other xylose-fermenting yeasts.
Agid:
23905
Handle:
10113/23905