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Application of immobilized tannase from Aspergillus niger for the removal of tannin from myrobalan juice
- Srivastava, Anita, Kar, Rita
- Indian journal of microbiology 2010 v.50 no.Supplement 1 pp. 46-51
- Aspergillus niger, Phyllanthus emblica, antioxidant activity, ascorbic acid, enzyme activity, immobilized enzymes, juices, pH, sodium alginate, submerged fermentation, tannase, temperature
- Tannase produced optimally on an agroresidue by an Aspergillus niger isolate under submerged fermentation immobilized on sodium alginate beads with 93.6% efficiency was applied for tannin removal from myrobalan/aonla (Phyllanthus emblica) juice. The pH and temperature optima of the immobilized enzyme were found to be 5.4 and 40°C while the corresponding values of the soluble enzyme were 5.8 and 35°C. Maximum tannin removal of 73.6% was obtained at 40°C and 150 rpm in 180 min with 36.6 U/ml of immobilized enzyme while the same amount of the soluble enzyme removed 45.2% of tannin at 37°C and 150 rpm in the same time period. The immobilized beads could be used repeatedly till 7th cycle with 77% efficiency. When preserved at 6°C the beads retained 71.7% of enzyme activity after 60 days. Reduction in vitamin C content, which is responsible for antioxidant property of the fruit, was minimum at only 2% during the treatment.