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Performance of a commercially available in-clinic ELISA for detection of antibodies against Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis, and Ehrlichia ewingii and Dirofilaria immitis antigen in dogs
- Stillman, Brett A., Monn, Michael, Liu, Jiayou, Thatcher, Brendon, Foster, Paulette, Andrews, Blaine, Little, Susan, Eberts, Matt, Breitschwerdt, Edward B., Beall, Melissa J., Chandrashekar, Ramaswamy
- Journal of the American Veterinary Medical Association 2014 v.245 no.1 pp. 80-86
- Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Dirofilaria immitis, Ehrlichia canis, Ehrlichia ewingii, antibodies, antigens, blood serum, cross reaction, dogs, enzyme-linked immunosorbent assay, heartworms, pathogens, screening
- Objective- To evaluate the performance of an in-clinic ELISA designed for detection of heartworm antigen and antibodies against 5 tick-borne pathogens. Design- Validation study.Sample-1,601 serum or matched serum, plasma, and blood samples from dogs. Results- Sensitivity and specificity of the in-clinic ELISA were > 89% for detection of antibodies against A phagocytophilum (93.2% and 99.2%, respectively), A platys (89.2% and 99.2%, respectively), B burgdorferi (96.7% and 98.8%, respectively), E canis (97.8% and 92.3%, respectively), and E ewingii (96.5% and 93.9%, respectively). Sensitivity of the assay for detection of D immitis was 98.9%, with 99.3% specificity. The in-clinic ELISA identified exposure to > 1 vector-borne pathogen in 354 of 1,195 samples. Cross-reactivity of E canis antigens with anti–E chaffeensis antibodies was confirmed. Results of field evaluations confirmed that the in-clinic ELISA could be reliably used under typical clinical conditions to identify dogs exposed to the pathogens of interest. Conclusions and Clinical Relevance- The in-clinic ELISA provided a comprehensive in-house serologic screening test for all vector-borne pathogens evaluated.