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Discrimination of sister-line IR rice varieties by polyacrylamide gel electrophoresis and reversed-phase high-performance liquid chromatography
- Huebner, F.R., Hussain, A., Lookhart, G.L., Bietz, J.A., Bushuk, W., Juliano, B.O.
- Cereal chemistry 1991 v.68 no.6 pp. 583
- Oryza sativa, dwarf cultivars, lines, line differences, genetic variation, detection, polyacrylamide gel electrophoresis, prolamins, glutenins, protein content, protein composition, high performance liquid chromatography
- Methods developed to identify genetically diverse rice varieties were applied to semidwarf rice varieties from the International Rice Research Institute derived from the same cross, which are especially difficult to differentiate. Acid polyacrylamide gel electrophoresis (PAGE) and Supelcosil C8 reversed-phase high-performance liquid chromatography (RP-HPLC) of 5 M acetic acid extracts of brown and milled rice and Vydac C18 RP-HPLC of 60% 1-propanol (prolamin) extracts of milled rice differentiated sister lines IR36 and IR42 (but not sister lines IR28 and IR29); IR32, IR38, and IR40; IR52 and IR54; and IR56 and IR60. Vydac C4 RP-HPLC of alkylated glutelins from milled rice differentiated IR36 from IR42 and IR52 from IR54 and showed slight differences between IR28 and IR29 and between IR38 and IR40. Vydac C4 RP-HPLC patterns of prolamins differentiated IR28 from IR29 and IR38 from IR40. Thus, PAGE and RP-HPLC (especially the latter) are useful for differentiating sister-line rice varieties. The effect of location of growth on brown rice PAGE, Vydac C18 RP-HPLC of milled rice prolamin, and Vydac C4 RP-HPLC of milled rice alkylated glutelins of IR36 and IR42 grown in three locations in the Philippines was quantitative rather than qualitative.