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Competitive exclusion of heterologous Campylobacter spp. in chicks
- Chen, H.C., Stern, N.J.
- Applied and environmental microbiology 2001 v.67 no.2 pp. 848
- chicks, Campylobacter jejuni, cecum, cultured cells, oral administration, strains, humans, interspecific competition, genes, bacterial proteins, polymerase chain reaction, restriction fragment length polymorphism, flagellin
- Chicken and human isolates of Campylobacter jejuni were used to provide oral challenge of day-old broiler chicks. The isolation ratio of the competing challenge strains was monitored and varied, depending upon the isolates used. A PCR-restriction fragment length polymorphism assay of the flagellin gene (flaA) was used to discriminate between the chick-colonizing isolates. Our observations indicated that the selected C. jejuni colonizers dominated the niche provided by the chicken ceca. Chicken isolates from the flaA type 7 grouping generally had numerical superiority over the human isolates when they were administered in our 1-day-old chick model. Our results suggest that it is possible to use combinations of C. jejuni chicken isolates as a defined bacterial preparation for the competitive exclusion of human-pathogenic C. jejuni in poultry.