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Effect of iron limitation on protein composition and ultrastructure of Haemophilus ducreyi
- Abeck, D., Johnson, A.P., Korting, H.C., Stolz, W., Struck‐Sauer, E., Braun‐Falco, O.
- FEMS microbiology letters 1990 v.68 no.1‐2 pp. 41-44
- Haemophilus ducreyi, chelating agents, cytoplasm, deferoxamine, gel electrophoresis, protein composition, proteins, siderophores, ultrastructure
- Protein profiles of whole cells of Haemophilusducreyi grown in the presence or absence of the iron chelator desferal, were compared by polycrylamide gel electrophoresis. Each of four strains produced novel proteins in the range 43–160 kDa when cultured under conditions of reduced iron availability. At some sub‐inhibitory concentrations, desferal produced enhanced growth, possibly due to it functioning as an exogenous siderophore. Organisms grown under conditions of reduced iron availability ultrastructurally showed also large periplasmic spaces between cytoplasm and outer membrane.