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Enhanced production of gamma-cyclodextrin from corn syrup solids by means of cyclododecanone as selective complexant

Author:
Rendleman, J.A. Jr.
Source:
Carbohydrate research 1993 v.247 pp. 223
ISSN:
0008-6215
Subject:
corn syrup, dextrins, ketones, maltose, glucose
Abstract:
In the presence of cyclododecanone as complexant, corn syrup solids (dextrose equivalent, d.e., 25; dp 4.5) were converted into cyclomaltooctaose (gamma-cyclodextrin, gamma-CD) in 28% yield by incremental addition of cyclodextrin glucanotransferase (CGTase) at 60 degrees C and pH 7.2. Cyclotridecanone was less effective in enhancing gamma-CD yield (14%); cyclic complexants with fewer than 12 ring atoms or more than 13 ring atoms were ineffective. In systems containing cyclododecanone, D-glucose strongly inhibited the conversion of both maltodextrin (starch hydrolyzate of dp 22) and corn syrup solids (dp 5). Maltose, when present in large proportion, also decreased yields of gamma-CD from corn syrup solids, but not from maltodextrin. Maltotriose had no inhibiting effect on either substrate. The nature of the high-molecular-weight fraction (dp > 10) of starch hydrolyzates was found to influence gamma-CD production and was the predominant factor causing yields from corn syrup solids to be lower than those from maltodextrin. Maltose itself did not undergo conversion: however, other low-molecular-weight maltooligosaccharides were converted into gamma-CD in good yield when treated incrementally with CGTase in the presence of cyclododecanone: 20.1% from maltotriose, 36.5% from maltotetraose, 44.1% from maltopentaose, 41.0% from maltohexaose, and 34.7% from maltoheptaose. Yields from maltooligosaccharides were adversely affected by the presence of both D-glucose and maltose.
Agid:
24933
Handle:
10113/24933