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Membrane topology of the GltS Na⁺/glutamate permease of Escherichia coli
- Szvetnik, Attila, Gál, József, Kálmán, Miklós
- FEMS microbiology letters 2007 v.275 no.1 pp. 71-79
- Escherichia coli, alkaline phosphatase, beta-galactosidase, genes, glutamic acid, models, sodium, symporters, topology, translation (genetics)
- The GltS Na⁺/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate : Na⁺ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and β-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.