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Membrane topology of the GltS Na⁺/glutamate permease of Escherichia coli

Author:
Szvetnik, Attila, Gál, József, Kálmán, Miklós
Source:
FEMS microbiology letters 2007 v.275 no.1 pp. 71-79
ISSN:
0378-1097
Subject:
Escherichia coli, alkaline phosphatase, beta-galactosidase, genes, glutamic acid, models, sodium, symporters, topology, translation (genetics)
Abstract:
The GltS Na⁺/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate : Na⁺ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and β-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.
Agid:
2537903