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The salt-induced ABC transporter Ota of the methanogenic archaeon Methanosarcina mazei Gö1 is a glycine betaine transporter

Author:
Schmidt, Silke, Pflüger, Katharina, Kögl, Stephanie, Spanheimer, Regina, Müller, Volker
Source:
FEMS microbiology letters 2007 v.277 no.1 pp. 44-49
ISSN:
0378-1097
Subject:
ABC transporters, Escherichia coli, Methanosarcina mazei, Western blotting, adenosine triphosphate, betaine, genes, methanogens, mutants, salinity, sugars
Abstract:
The genes encoding the three subunits of the primary ABC transporter Ota of the methanogenic archaeon Methanosarcina mazei Gö1 were cloned in an expression vector (pBAD24) and transformed into the glycine betaine transport-negative mutant Escherichia coli MKH13. Ota was produced as demonstrated by Western blotting. Uptake studies revealed that Ota catalyzed the transport of glycine betaine in E. coli MKH13(pBAD-Ota) with a Km of 10±5 μM and a maximal velocity of 1.5±0.5 nmol min⁻¹ mg protein⁻¹. Transport was ATP dependent. Ota was activated by salinity gradients, but only marginally by sugar gradients across the membrane. Glycine betaine transport was inhibited to a small extent by an excess of dimethylglycin or proline betaine, but not by sarcosine or glycine.
Agid:
2537931