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Identifying differentially expressed genes in the dimorphic fungus Penicillium marneffei by suppression subtractive hybridization

Liu, Hongfang, Xi, Liyan, Zhang, Junmin, Li, Xiqing, Liu, Xingyin, Lu, Changming, Sun, Jiufeng
FEMS microbiology letters 2007 v.270 no.1 pp. 97-103
Penicillium, cDNA libraries, cell cycle, cell walls, complementary DNA, dimorphism, gene expression, gene expression regulation, genes, humans, metabolism, mycelium, pathogenicity, reverse transcriptase polymerase chain reaction, signal transduction, stress response, suppression subtractive hybridization, yeasts
Penicillium marneffei is the only known Penicillium species that exhibits temperature-dependent dimorphic growth. It usually infects immunocompromised individuals and may cause fatal penicilliosis marneffei. Its pathogenicity appears to be intimately related to the dimorphic transition from a mycelial phase in the environment to a yeast phase in the human host. To further understand the molecular mechanisms in the switching process of Penicillium marneffei, differential gene expression analysis was carried out between the mycelial phase and the yeast phase by suppression subtractive hybridization. Five hundred genes from forward and reverse-subtracted cDNA libraries were screened by cDNA array dot blotting and 43 genes were identified to be differentially expressed. These genes were found to be homologs of genes involved in various cellular processes including cell wall synthesis, signal transduction, cell cycle, substance transport, general metabolism and stress response, etc. Six genes were further analyzed using relative quantification real-time reverse transcriptase PCR. It is believed that these genes determine some of the major characteristics of dimorphism in Penicillium marneffei.