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Selection of a mutation conferring high NaCl tolerance to gametophytes of Ceratopteris

Hickok, L.G., Vogelien, D.L., Warne, T.R.
Theoretical and applied genetics 1991 v.81 no.3 pp. 293-300
salt tolerance, spores, Ceratopteris richardii, developmental stages, mutagenesis, sodium chloride, spore germination, incomplete dominance, artificial selection
Spores from a weakly salt tolerant strain of Ceratopteris richardii containing the mutation stl1 were irradiated and sown on nutrient medium supplemented with 200 mM NaCl. A single highly salt tolerant gametophyte was recovered and selfed to generate a homozygous sporophyte. Spores from this strain, 10 alpha 23, were used to document the sexual transmission of the trait and to monitor the inheritance of tolerance in crosses to both the wild type and to the parental salt tolerant strain. Genetic analysis showed the 10 alpha 23 strain to possess both the original stl1 mutation and an additional semi-dominant nuclear mutation, stl2, that individually conferred a high level of tolerance to gametophytes. In combination, both mutations had additive effects. Tolerance was also evident in sporophytes, but at a lower level than in gametophytes.