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Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway

Chen, Chen, Jiang, Zhen-You, Yu, Bin, Wu, Xian-Lin, Dai, Cong-Qi, Zhao, Chang-Lin, Ju, Da-Hong, Chen, Xiao-Yin
Journal of Asian natural products research 2012 v.14 no.9 pp. 877-885
Toll-like receptor 7, Western blotting, blood, cell death, cell viability, coculture, dendritic cells, epithelial cells, gene expression, genes, granulocyte-macrophage colony-stimulating factor, humans, interleukin-4, macrophages, models, oseltamivir, quantitative polymerase chain reaction, quercetin, reverse transcriptase polymerase chain reaction, signal transduction, umbilical cord, viruses
The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1. After immunological cells and virus-infected cells were co-cultured, quercetin and oseltamivir were also added into the medium as a treatment intervention. Then the immunological cells were collected for Real Time PCR (RT-PCR) and Western blot to determine the expression levels of genes related to TLR7 pathway. Viral infection led to cell death and increased the gene expression levels of TLR7 signal pathway. Quercetin and oseltamivir increased cell viability and reduced the expression levels of TLR7 signal pathway.