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Antimicrobial activities of phenethyl isothiocyanate isolated from horseradish

Author:
Chen, Hongxia, Wang, Chengzhang, Ye, Jianzhong, Zhou, Hao, Chen, Xijuan
Source:
Natural product research 2012 v.26 no.11 pp. 1016-1021
ISSN:
1478-6427
Subject:
Cytospora, Fusarium graminearum, Phytophthora, Xanthomonas axonopodis pv. citri, anti-infective properties, chromatography, filtration, horseradish, nuclear magnetic resonance spectroscopy, petroleum, silica gel
Abstract:
Phenethyl isothiocyanate (PEITC) was obtained from horseradish. The preparation procedure was as follows: the horseradish powder was hydrolysed in the water first, and then, after filtration, the residue was extracted by petroleum ether; finally, PEITC was isolated by silica gel column. The structure of PEITC was identified by IR, MS, ¹H-NMR and ¹³C-NMR chromatography methods. The inhibitory activities of PEITC against Gibberella zeae, Xanthomonas axonopodis pv. citri, Cytospora sp. and Phytophthora capsisi showed that PEITC had good inhibition effects. The EC₅₀ values of G. zeae, X. axonopodis pv. citri, Cytospora sp. and P. capsisi were 13.92, 1.20, 0.73 and 3.69 µg mL⁻¹, respectively.