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Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi)

Author:
Li, Xican, Chen, Dongfeng, Mai, Ying, Wen, Bi, Wang, Xiaozhen
Source:
Natural product research 2012 v.26 no.11 pp. 1050-1053
ISSN:
1478-6427
Subject:
2,2-diphenyl-1-picrylhydrazyl, antioxidant activity, antioxidants, correlation, flavonoids, polysaccharides, saponins, sugars
Abstract:
Five Radix Astragali (RA) extracts were prepared and their antioxidant activities were measured in vitro using ABTS [2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid diammonium salt)], DPPH · (1,1-diphenyl-2-picrylhydrazyl radical), reducing power, and •OH assays. Their chemical contents were then determined, including total phenolics, total flavonoids, total saponins and total sugars. The 1/IC₅₀ values of the various antioxidant assays were used to evaluate the level of antioxidant activity of the RA extracts (plot showing 1/IC₅₀ values vs. chemical contents), and the average R values (correlation coefficients) of total phenolics and total flavonoids were 0.762 and 0.638. In contrast, the average R values of the total saponins and total sugars were −0.0386 and −0.132, respectively. This large difference clearly demonstrates that the antioxidant effects of RA in vitro might be generally considered to be a result of the presence of phenolic compounds (including flavonoids) but not astragalosides and polysaccharides.