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Antioxidant, xanthine oxidase and lipoxygenase inhibitory activities and phenolics of Bauhinia rufescens Lam. (Caesalpiniaceae)

Author:
Compaoré, M., Lamien, C.E., Lamien-Meda, A., Vlase, L., Kiendrebeogo, M., Ionescu, C., Nacoulma, O.G.
Source:
Natural product research 2012 v.26 no.11 pp. 1069-1074
ISSN:
1478-6427
Subject:
2,2-diphenyl-1-picrylhydrazyl, Bauhinia, acetone, antioxidant activity, antioxidants, enzyme inhibitors, ethyl acetate, ferulic acid, high performance liquid chromatography, hydrolysis, isoquercitrin, kaempferol, leaves, lipoxygenase, mass spectrometry, quercetin, quercitrin, rutin, xanthine oxidase
Abstract:
An aqueous acetone extract of the stem with the leaves of Bauhinia rufescens and its fractions were analysed for their antioxidant and enzyme-inhibitory activities, as well as their phytochemical composition. For measurement of the antioxidant activities, the 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azinobis(3-ethylbenzoline-6-sulphonate) and the ferric-reducing methods were used. The results indicated that the aqueous acetone, its ethyl acetate and n-butanol fractions possessed considerable antioxidant activity. Further, the xanthine oxidase and lipoxygenase inhibitory assays showed that the n-butanol fraction possessed compounds that can inhibit both these enzymes. In the phytochemical analysis, the ethyl acetate and the n-butanol fractions of the aqueous acetone extract were screened by HPLC–MS for their phenolic content. The results indicated the presence of hyperoside, isoquercitrin, rutin quercetin, quercitrin, p-coumaric and ferulic acids in the non-hydrolysed fractions. In the hydrolysed fractions, kaempferol, p-coumaric and ferulic acids were identified.