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Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'
- Kurtzman, Cletus P., Robnett, Christie J., Basehoar-Powers, Eleanor
- FEMS yeast research 2001 v.1 no.2 pp. 133
- Zygosaccharomyces, new species, new geographic records, yeasts, starter cultures, ribosomal DNA, internal transcribed spacers, sequence analysis, molecular systematics, phylogeny, fungal anatomy, fungus physiology, fermented foods, tea (beverage), food microbiology
- A new ascosporogenous yeast, Zygosaccharomyces kombuchaensis sp. n. (type strain NRRL YB-4811, CBS 8849), is described; it was isolated from Kombucha tea, a popular fermented tea-based beverage. The four known strains of the new species have identical nucleotide sequences in domain D1/D2 of 26S rDNA. Phylogenetic analysis of D1/D2 and 18S rDNA sequences places Z. kombuchaensis near Zygosaccharomyces lentus. The two species are indistinguishable on standard physiological tests used for yeast identification, but can be recognized from differences in restriction fragment length polymorphism patterns obtained by digestion of 18S-ITS1 amplicons with the restriction enzymes DdeI and MboI.