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Gene expression during induction of somatic embryogenesis in carrot cell suspensions
- Aleith, F., Richter, G.
- Planta 1991 v.183 no.1 pp. 17-24
- Daucus carota, somatic embryogenesis, embryo (plant), gene expression, genes, messenger RNA, complementary DNA, nucleotide sequences, plant proteins, molecular conformation, cell suspension culture, amino acid sequences, transcription (genetics), protein secondary structure
- We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDNA library (lambda gt 10) was established using poly(A)+-rich RNAs from cells deprived of auxin for 8 d. By differential screening a number of clones corresponding to early-induced embryogenic genes were identified. For several a temporary accumulation of the specific mRNA between 6 and 16 d after induction was observed. With regard to the nucleotide sequence and the respective deduced amino-acid sequence, two glycine-rich proteins and a polypeptide with a proline-rich domain were among the products of genes activated at the onset of somatic embryogenesis.