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Separation of oxidized and unoxidized molecular species of phosphatidylcholine by high pressure liquid chromatography
- Crawford, C.G., Plattner, R.D., Sessa, D.J., Rackis, J.J.
- Lipids 1980 v.15 no.2 pp. 91
- oxidation, separation, high performance liquid chromatography, phosphatidylcholines, soybeans, methanol, antioxidants
- Soy phosphatidylcholine (PC) has been separated into its major molecular species by reversephase high pressure liquid chromatography (HPLC). An aqueous methanol gradient was used that allowed detection of the various species by their absorbance at 206 nm. Oxidized species were detected by their absorbance at 234 nm and were resolved from the unoxidized species. This technique has been used to separate and purify unoxidized dilinoleyl phosphatidylcholine (di 18:2 PC) from other species of soy PC and to monitor the autoxidation of an aqueous suspension of the purified di 18:2 PC. Two oxidized products were formed from di 18:2 PC. Further analysis showed that they were PC, but one of the products contained an oxidized and an unoxidized fatty acid; in the other product, both fatty acids were oxidized.