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Action patterns of immobilized dextranase
- Smiley, K.L., Boundy, J.A., Hensley, D.E.
- Carbohydrate research 1982 v.104 no.2 pp. 319
- immobilized enzymes, dextranase, Penicillium funiculosum, Penicillium, enzyme activity, dextran, enzymatic hydrolysis, Leuconostoc mesenteroides, oligosaccharides, molecular weight
- Dextranase, isolated from Penicillium funiculosum and P. lilacinum, was immobilized on porous, silanized-silica beads and a phenol-formaldehyde resin. A commercial dextran of relatively low molecular weight (∼2 × 106) was degraded by immobilized dextranase, with the formation of reducing sugars, but with little decrease in viscosity. In contrast, soluble dextranase caused rapid loss of viscosity, but only a slight increase in reducing sugar. Native dextran of high molecular weight, from Leuconostoc mesenteroides NRRL B-512 (F), was attacked very slowly by immobilized dextranase, with the release of oligosaccharides of low molecular weight.