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Detection and variability of the lethal yellowing group (16Sr IV) phytoplasmas in the Cedusa sp. (Hemiptera: Auchenorrhyncha: Derbidae) in Jamaica

Brown, S.E., Been, B.O., McLaughlin, W.A.
Annals of applied biology 2006 v.149 no.1 pp. 53-62
Derbidae, Hemiptera, Phytoplasma, coconuts, genes, lethal yellowing, polymerase chain reaction, restriction fragment length polymorphism, ribosomal DNA, ribosomal RNA, sequence analysis, Florida, Jamaica
Lethal yellowing (LY) group phytoplasma was detected in members of the Cedusa species of Derbids from Jamaica by nested PCR assay employing the rRNA primer pairs P1/P7 and LY16Sf/LY16Sr. A 1400-bp product was obtained from 13/43 Derbids that were analysed. Restriction fragment length polymorphism analysis of the nested PCR product revealed variations in 6/13 Derbids analysed, suggesting that the planthoppers could be infected with different strains of the LY group phytoplasma. Sequence analysis of the 16S rDNA gene determined that the percentage of similarity of the phytoplasma strains in the Derbids ranged from 97-98% to the phytoplasma strain found in coconuts in Florida (LYFL-C2) and Jamaica (LYJ-C8). The phytoplasma strains in the Derbids clustered together with the Jamaican and Florida coconut LY phytoplasma, as well as other characterised strains composing the LY phytoplasma (16Sr IV) group.