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Levels of Lipid Peroxidation in Human Plasma and Erythrocytes: Comparison between Fatty Acids and Cholesterol

Author:
Yoshida, Yasukazu, Saito, Yoshiro, Hayakawa, Mieko, Habuchi, Yoko, Imai, Yasuharu, Sawai, Yoshiyuki, Niki, Etsuo
Source:
Lipids 2007 v.42 no.5 pp. 439-449
ISSN:
0024-4201
Subject:
antioxidants, biomarkers, cholesterol, erythrocytes, fatty acids, humans, lipid peroxidation, metabolism, oxidants, oxidative stress, potassium hydroxide, saponification, sodium, toxicity
Abstract:
Lipid peroxidation has gained renewed attention with increasing evidence showing its biological role in producing toxic compounds and cellular signaling mediators. The assessment of lipid peroxidation levels in vivo is difficult partly because lipids are oxidized by different oxidants by different mechanisms to give versatile types of products, which may undergo metabolism and secondary reactions. In the present study, total hydroxyoctadecadienoic acids (tHODE) and 7α- and 7β-hydroxycholesterol (t7-OHCh) from 44 healthy human subjects were assessed as biomarkers after reduction with sodium borohydride followed by saponification with potassium hydroxide comparing with the prevailing standard 8-isoprostaglandin F₂α (t8-iso-PGF₂α). The average concentrations of tHODE, total 8-isoprostaglandin F₂α (t8-iso-PGF₂α), t7α-OHCh, and t7β-OHCh were 203, 0.727, 87.1, and 156 nmol/l plasma and 1,917, 12.8, 1,372, and 3,854 nmol/l packed erythrocytes, respectively. The ratios of tHODE and t7-OHCh to the parent substrates were 194 and 3,519 μmol tHODE/mol linoleates and 40.9 and 686 μmol t7-OHCh/mol cholesterol in plasma and erythrocytes, respectively. It was found that (1) t7-OHCh in blood was unexpectedly high, as high as or even higher than tHODE, (2) the amounts of tHODE was more than 100 fold higher than t8-iso-PGF₂α (3) the level of lipid oxidation products in erythrocytes was higher than that in plasma, and (4) lipid peroxidation products level tended to increase while antioxidant level decrease with age. These products may be used as potential biomarker for assessment of lipid peroxidation and oxidative stress in vivo.
Agid:
271871