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The roles of the proteasome, and cathepsins B, L, H and D, in ostrich meat tenderisation

Author:
Thomas, A.R., Gondoza, H., Hoffman, L.C., Oosthuizen, V., Naude, R.J.
Source:
Meat science 2004 v.67 no.1 pp. 113-120
ISSN:
0309-1740
Subject:
ostriches, poultry meat, food storage, storage temperature, storage time, postmortem changes, myofibrils, cathepsins, protein degradation, proteolysis, enzyme activity, tenderizing, meat tenderness, meat quality
Abstract:
As very little research has been conducted on ostrich meat tenderisation, this study aims at investigating the roles of the proteasome and cathepsins B, L, H, and D in the tenderisation process. The enzyme activities in meat from eight ostriches during a 12-day ageing period and the corresponding physical characteristics (e.g. pH, shear force) and myofibril patterns were determined. After 12 days, substantial high remaining activities were found, especially of the proteasome, thus implicating their possible roles in the tenderisation process. The mean shear force values, however, showed no improvement in tenderness, but the myofibril patterns showed the appearance of a M(r) 32 K component. Myofibril degradation studies of the proteasome, analysed electrophoretically, also revealed a possible role of the proteasome, but under activating conditions. This study provides further insights into the tenderisation process, particularly of ostrich meat, which may ultimately be used for the advantageous manipulation of the process.
Agid:
2723404