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Effect of several factors on peracetic acid pretreatment of sugarcane bagasse for enzymatic hydrolysis

Zhao, Xue-bing, Wang, Lei, Liu, De-hua
Journal of chemical technology and biotechnology 2007 v.82 no.12 pp. 1115-1121
cellulose, endo-1,4-beta-glucanase, enzymatic hydrolysis, glucose, hemicellulose, lignin, lignocellulose, oxidants, peracetic acid, sodium hydroxide, sugarcane, sugarcane bagasse, sulfuric acid, temperature
BACKGROUND: Lignocellulose should undergo pretreatment to enhance its enzymatic digestibility before being saccharified. Peracetic acid (PAA) is a strong oxidant that can remove lignin under mild conditions. The sulfuric acid in the PAA solution also can cause degradation of hemicelluloses. The objective of the present work is to investigate the effect of several factors on peracetic acid pretreatment of sugarcane bagasse.RESULTS: It was found that PAA charge, liquid/solid (l/s) ratio, temperature, time, interactions between PAA charge and l/s ratio, temperature and time, all had a very significant effect on the enzymatic conversion ratio of cellulose. The relative optimum condition was obtained as follows: PAA charge 50%, l/s ratio 6:1, temperature 80 °C and time 2 h. More than 80% of the cellulose in bagasse treated under the above conditions was converted to glucose by cellulase of 20 FPU g⁻¹ cellulose. Compared with H₂SO₄ and NaOH pretreatments under the same mild conditions, PAA pretreatment was the most effective for enhancement of enzymatic digestibility.CONCLUSION: PAA pretreatment could greatly enhance the enzymatic digestibility of sugarcane bagasse by removing hemicelluloses and lignin, but removal of lignin was more helpful. This study can serve as a step to further optimization of PAA pretreatment and understanding the mechanism of enhancement of enzymatic digestibility.