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Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode for the separation of purines and pyrimidines
- Kažoka, H.
- Journal of chromatography 2008 v.1189 no.1-2 pp. 52-58
- adsorption, liquid chromatography, purines, pyrimidines, silica, solutes
- The diol-bonded phase (column LiChrospher 100 Diol) has been studied for the separation of some purines and pyrimidines under normal-phase liquid chromatography (NPLC) conditions. Retention time, column efficiency, and selectivity of column with diol-phase were compared to those of unmodified silica (column LiChrospher SI-60). It was established that under adsorption NPLC mode application of diol-phase can reduce the separation time and a little improve the column efficiency. Significant improvement of the column efficiency for polar solutes is observed if mixed partition-adsorption NPLC mode is used. The investigation has shown that application of diol-phase instead of bare silica is useful not only under adsorption, but also under mixed partition-adsorption mode if the system with specific selectivity is necessary.