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Role of antimicrobial agents in simultaneous saccharification and fermentation of paddy malt mash to ethanol by mixed cultures of Saccharomyces cerevisiae PHO3 and Zymomonas mobilis ZM4
- Agrawal, R., Basappa, S.C.
- Biotechnology letters 1996 v.18 no.6 pp. 673-678
- Saccharomyces cerevisiae, Zymomonas mobilis, amylases, benzylpenicillin, coculture, ethanol, fermentation, glucose, kanamycin, malt, mash, mixed culture, pH, plant extracts, polymyxins, rice, saccharification, streptomycin, tetracycline
- Among various antimicrobial plant extracts, chemicals and antibiotics used for simultaneous saccharification and fermentation, penicillin G prevented contamination and did not inhibit amylase activity and growth of the synergistic co-cultures Saccharomyces cerevisiae PH03 and Zymomonas mobilis ZM4 during a 7-day fermentation of paddy malt (25.0%) mash (18.0% dextrose equivalent) to ethanol at 30 degrees C and pH 5.5. The treatment yielded 10.1% (v/v) ethanol from the mash which was significantly more than that of the boiled and fermented mash (9.3% v/v) and equal to that of the mash boiled and fermented (10.2% v/v) after added amylases treatment. Most of the other compounds (kanamycin, streptomycin, polymyxin, tetracycline) had growth inhibitory effect especially on Z. mobilis.