Jump to Main Content
Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast
- Cao, N.J., Krishnan, M.S., Du, J.X., Gong, C.S., Ho, N.W.Y., Chen, Z.D., Tsao, G.T.
- Biotechnology letters 1996 v.18 no.9 pp. 1013-1018
- Saccharomyces, acetates, ammonia, ammonium hydroxide, batch fermentation, biomass, cellulose, corn cobs, ethanol, ethanol production, genetic engineering, genetically engineered microorganisms, glucose, lignin, saccharification, soaking, xylose
- A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylose-fermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.