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Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast

Cao, N.J., Krishnan, M.S., Du, J.X., Gong, C.S., Ho, N.W.Y., Chen, Z.D., Tsao, G.T.
Biotechnology letters 1996 v.18 no.9 pp. 1013-1018
Saccharomyces, acetates, ammonia, ammonium hydroxide, batch fermentation, biomass, cellulose, corn cobs, ethanol, ethanol production, genetic engineering, genetically engineered microorganisms, glucose, lignin, saccharification, soaking, xylose
A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylose-fermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.