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Metabolism of meta- or/and para-chlorinated benzoic acids by the crude extract of Alcaligenes sp. strain CPE3 grown on 3,4-dichlorobenzoic acid
- Fava, F., Baldoni, F., Marchetti, L.
- Biotechnology letters 1996 v.18 no.9 pp. 1007-1012
- Alcaligenes, NAD (coenzyme), benzoic acids, enzyme kinetics, metabolism, oxygen
- A crude extract of Alcaligenes sp. CPE3 strain grown on 3,4-dichlorobenzoic acid metabolised 3- and 4-chlorobenzoic acid by reactions requiring O2 and NADH, and 3,4-dichlorobenzoic acid by a reaction requiring O2, NADH, FAD and FMN. The specific activity of the extract vs. 3-chlorobenzoic acid was described by the Michaelis-Menten kinetics, that vs. 4-chlorobenzoic acid was described by a substrate inhibitory kinetics and that vs. 3,4-dichlorobenzoic acid exhibited a two-peaked profile.