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Enhancement of plasmid stability and enzymatic expression by immobilising recombinant Saccharomyces cerevisiae
- Lu Chau, T., Guillan, A., Roca, E., Nunez, M.J., Lema, J.M.
- Biotechnology letters 2000 v.22 no.15 pp. 1247-1250
- Saccharomyces cerevisiae, calcium alginate, enzyme stability, gels, genes, plasmids, protein synthesis, recombinant proteins
- Immobilisation of cells in a calcium alginate gel improved plasmid stability (up to 50%) and enzymatic expression (up to 57%) of a recombinant Saccharomyces cerevisiae over-expressing the homologous gene EXG1. The rate of segregational loss in the free cells was 14-fold higher than that of the immobilised cells. Recombinant protein synthesis requires reduced cofactors, which affect the redox balance of the cell.