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Identification of RFLP markers linked to the unifoliate leaf, cauliflower head mutation of alfalfa
- Brouwer, D.J., Osborn, T.C.
- Journal of heredity 1997 v.88 no.2 pp. 150-152
- alfalfa, cauliflower, chromosome mapping, clones, diploidy, flowers, genetic markers, leaves, linkage groups, loci, restriction fragment length polymorphism
- The cauliflower head and unifoliate leaf mutation (uni) is a unique developmental mutation of alfalfa that may shed some light on the relationship between leaves and floral organs. RFLP loci linked to uni were identified using bulk segregant analysis of an F2 diploid alfalfa population segregating for this trait. Two bulks, one unifoliate leaf and one trifoliate leaf, were formed with each composed of 20 F2 plants and screened using 110 RFLP clones. Seven clones detected polymorphisms between the bulks. Linkage analysis confirmed that three of the clones were tightly linked to uni and located on linkage group 4 of a previously published linkage map. The clones provide useful tools for future genetic and molecular studies.