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In vitro and in vivo tumor suppressive activity induced by human telomerase transcriptase-targeting antisense oligonucleotides mediated by cationic liposomes

Han, Min, Chen, Jin-Liang, Hu, Ying, He, Cai-Li, Shuai, Wu-Ping, Yu, Jian-Heng, Chen, Hai-Liang, Liang, Wen-Quan, Mayumi, Tadanori, Shinsaku, Nakagawa, Gao, Jian-Qing
Journal of bioscience and bioengineering 2008 v.106 no.3 pp. 243-247
adenocarcinoma, cell growth, cholesterol, fluorouracil, humans, inhibitory concentration 50, neoplasm cells, oligodeoxyribonucleotides, telomerase
The objective of this study was to investigate the in vitro and in vivo influence of cationic liposomes on the tumor suppressive effect of antisense telomerase oligodeoxynucleotides to human cervical adenocarcinoma cells (HeLa). Antisense oligodeoxynucleotides (ASODN) against the human telomerase transcriptase (hTERT) served as telomerase inhibitors. The cholesterol derivative, 3β [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), was synthesized, and cationic liposomes (CL) were prepared using DC-Chol and dioleoylphosphatidylethanolamine (DOPE). The in vitro IC50 of the CL-ASODN complex was 1.88 μmol/l, while the IC50 of the cells treated with free ASODNs or CL alone was 25.24 μmol/l and 55.18 μmol/l, respectively. The CL-ASODN complex inhibited HeLa cell growth for at least 120 h. In vivo, the CL-ASODN complex inhibited the tumor growth rate by 55.11%, which increased to 89.47% when CL-ASODN was combined with 5-fluorouracil treatment. ASODNs alone failed to induce tumor-suppressive activity, suggesting that CL prepared from DOPE and DC-Chol can significantly enhance the growth inhibitory effect of ASODN on tumor cells both in vitro and in vivo.