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The porcine skin associated T-cell homing chemokine CCL27: molecular cloning and mRNA expression in piglets infected experimentally with Staphylococcus hyicus
- Johnsen, Christina K., Jensen, Annette N., Ahrens, Peter, Andresen, Lars Ole
- Veterinary immunology and immunopathology 2003 v.96 no.1-2 pp. 13-18
- CCR10 receptor, Staphylococcus hyicus, T-lymphocytes, chemokine CCL27, complementary DNA, gene expression, gene expression regulation, humans, inflammation, keratinocytes, messenger RNA, mice, molecular cloning, piglets, tissues
- CCL27 (also named CTACK, ALP, ILC and ESkine) is a CC chemokine primarily expressed by keratinocytes of the skin. The cognate receptor of CCL27 named CCR10 (GPR-2), is also expressed in skin-derived cells, and in addition by a subset of peripheral blood T-cells and in a variety of other tissues. In this paper, we report the cloning of porcine CCL27 cDNA and investigation of CCL27 mRNA expression in Staphylococcus hyicus infected piglets. At the protein level, 77 and 74% homology was found to human and mouse CCL27 sequences, respectively. The results of the expression analyses show that CCL27 mRNA is upregulated in the skin of infected piglets and to a lesser extent in piglets recovered from disease and without clinical signs of infection, indicating a role for CCL27 both during inflammation and after recovery from an infection.