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Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues

Jaber, J.R., Fernández, A., Herráez, P., Monteros, A. Espinosa de los, Ramı́rez, G.A., Garcia, P.M., Fernandez, T., Arbelo, M., Perez, J.
Veterinary immunology and immunopathology 2003 v.96 no.1-2 pp. 65-72
Stenella coeruleoalba, T-lymphocytes, antibodies, antigen presentation, cattle, cortex, cross reaction, dolphins, hepatitis, humans, immunoglobulin G, liver, lymph nodes, lysozyme, macrophages, parasites, plasma cells
This study evaluates the cross-reactivity of seven anti-human and one anti-bovine antibodies in formalin-fixed, paraffin-embedded tissue samples of liver and mesenteric lymph nodes of 13 striped dolphins (Stenella coeruleoalba). Four antibodies (CD3, IgG, lysozyme and S100 protein) reacted with striped dolphin lymph nodes in a similar pattern to that observed in the species of origin. The anti-human MHC class II mAb reacted strongly with macrophages and dendritic-like cells of striped dolphins, whereas a small number of lymphocytes were labelled with this antibody. These antibodies were used to study the immunophenotype of the inflammatory infiltrated in non-specific chronic reactive hepatitis (eight cases) and chronic parasite cholangitis (two cases) and normal liver (three cases) of striped dolphins. Non-specific chronic reactive hepatitis was composed of inflammatory infiltration of CD3+ T lymphocytes and IgG+ plasma cells in portal spaces and hepatic sinusoids. Lymphonodular aggregates observed in chronic parasitic cholangitis showed a cellular distribution similar to that found in lymph node cortex, including the presence of S100+ and MHC class II+ dendritic-like cells in lymphoid follicles and interfollicular areas. This result suggests that those inflammatory infiltrates are highly organised to enhance antigen presentation to B and T cells.