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The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells
- Chibbar, R.N., Kartha, K.K., Datla, R.S.S., Leung, N., Caswell, K., Mallard, C.S., Steinhauer, L.
- Plant cell reports 1993 v.12 no.9 pp. 506-509
- Hordeum vulgare, genetic transformation, structural genes, introns, actin, alcohol dehydrogenase, Cauliflower mosaic virus, recombinant DNA, reporter genes, beta-glucuronidase, gene expression, transgenic plants, promoter regions, laboratory techniques
- The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcoholdehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intron1 along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli beta- glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoter- intron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.