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Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus
- Liu, Long, Du, Guocheng, Chen, Jian, Wang, Miao, Sun, Jun
- Bioprocess and biosystems engineering 2009 v.32 no.6 pp. 755-763
- Streptococcus equi subsp. zooepidemicus, agitation, dissolved oxygen, hyaluronic acid, models, oxygen, perfluorocarbons, shear stress
- Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach. In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0-8 h, 400 rpm during 8-12 h, and 600 rpm during 12-20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0-8 h and at 5% during 8-20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model. Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA production was achieved.