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Partial nitritation treatment of underground brine waste with high ammonium and salt content

Author:
Shinohara, Takehiko, Qiao, Sen, Yamamoto, Taichi, Nishiyama, Takashi, Fujii, Takao, Kaiho, Tatsuo, Bhatti, Zafar, Furukawa, Kenji
Source:
Journal of bioscience and bioengineering 2009 v.108 no.4 pp. 330-335
ISSN:
1389-1723
Subject:
ammonia, biomass, iodine, methane, methane production, microbial communities, nitrates, nitrification, nitrifying bacteria, nitrite nitrogen, nitrites, nitrogen, nitrogen-fixing bacteria, pH, ribosomal RNA, salinity, salt content, temperature, wastes, Japan
Abstract:
Underground brine waste containing high concentrations of ammonium and with a salinity of 3% is usually generated during the production of methane gas and iodine in the gas field of Chiba Prefecture, Japan. In this study, one swim-bed reactor, packed with a novel acrylic fiber biomass carrier (Biofringe), was applied to the partial nitritation treatment of this kind of underground brine waste. A stable nitrite production rate of 1.6 kg NO₂-N m⁻³ d⁻¹ was obtained under a nitrogen loading rate of 3.0 kg-N m⁻³ d⁻¹, at a pH of 7.5 and a temperature of 25 °C. Nitrate production was negligible and the effluent NO₂-N/NO x -N ratio was above 98% due to the successful inhibition of nitrite-oxidizing bacterial activity. Free ammonia was considered to be the main factor for inhibiting the activity of nitrite-oxidizing bacteria. A microbial community shift was demonstrated by 16S rRNA analysis, and it was shown that the ammonium-oxidizing bacteria became the predominant species after successful nitrite accumulation was observed.
Agid:
281408