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Tests for epi-progoitrin, derived nitriles, and goitrin in body tissues from cattle fed crambe meal
- VanEtten, C.H.
- Canadian journal of animal science 1977 v.57 no.1 pp. 75
- beef cattle, feed supplements, feed meals, crambe meal, tissue analysis, glucosinolates, nitriles, goitrin, detection, quantitative analysis, natural toxicants, cattle feeding
- Beef cattle were fed a ration formulated with up to 10% processed crambe [Crambe abyssinica] seed meal, which contained varying amounts of the glucosinolate epi-progoitrin (e-PG), unsaturated nitril [(S) 1-cyano-2-hydroxy-3-butene] and goitrin. Taken at commercial slaughter of the animals, samples of their fat from near the kidney, the kidney, liver and shoulder muscle tissues contained no detectable e-PG, unsaturated nitrile, episulfide nitriles [isomers 1-cyano-(S)-2-hydroxy-(R)- and (S)-3-epithiobutanes] or goitrin. Examination of GLC curves also showed absence of an isomer of goitrin, recently reported to be formed by the action of sheep rumen fluid on progoitrin. Details on the separation of the small amounts of active compounds from the body tissues and on their subsequent GLC analyses are presented. Detection was possible to levels below 1 ppm. Results from spiked body tissues show the sensitivity and the precision that can be expected.