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Development of a novel luminol chemiluminescent method catalyzed by gold nanoparticles for determination of estrogens

Li, Yongxin, Yang, Ping, Wang, Po, Wang, Lun
Analytical and bioanalytical chemistry 2007 v.387 no.2 pp. 585-592
X-radiation, X-ray photoelectron spectroscopy, chemiluminescence, detection limit, estradiol, estriol, estrone, nanogold, pregnant women, transmission electron microscopy, urine
It has been found that gold nanoparticles (nano-Au) enhance the chemiluminescence (CL) of the luminol-hydrogen peroxide system and that estrogens inhibit these CL signals in alkaline solution. CL spectra, UV-visible spectra, X-ray photoelectron spectra (XPS), and transmission electron microscopy (TEM) were used to investigate the mechanism of the CL enhancement. On the basis of the inhibition, a flow-injection CL method has been established for determination of three natural estrogens. Under the optimized conditions, the linear range for determination of the estrogens was 0.07 to 7.0 μmol L-¹ for estrone, 0.04 to 10 μmol L-¹ for estradiol, and 0.1 to 10 μmol L-¹ for estriol. The detection limits were 3.2 nmol L-¹ for estrone, 7.7 nmol L-¹ for estradiol, and 49 nmol L-¹ for estriol, with RSD of 2.9, 2.6, and 1.8%, respectively. This method has been used for analysis of estrogens in commercial tablets and in urine samples from pregnant women.