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Quantitative considerations for suspension array assays

Author:
Connolly, Ashley R., Palanisamy, Ramkumar, Trau, Matt
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 17-22
ISSN:
0168-1656
Subject:
DNA, RNA, biotechnology, gene expression regulation, macrophages
Abstract:
A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 10⁸ copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting.
Agid:
282642