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Preparation and characterization of folate-poly(ethylene glycol)-grafted-trimethylchitosan for intracellular transport of protein through folate receptor-mediated endocytosis

Author:
Zheng, Yu, Song, Xiangrong, Darby, Michael, Liang, Yufeng, He, Ling, Cai, Zheng, Chen, Qiuhong, Bi, Yueqi, Yang, Xiaojuan, Xu, Jiapeng, Li, Yuanbo, Sun, Yiyi, Lee, Robert J., Hou, Shixiang
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 47-53
ISSN:
0168-1656
Subject:
biopharmaceuticals, biotechnology, bovine serum albumin, composite polymers, electrolytes, endocytosis, ethylene glycol, fluorescein, folic acid, neoplasm cells
Abstract:
To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) were prepared under suitable weight ratio of copolymer to FITC-BSA by ionic interaction between the positively charged copolymers and the negatively charged FITC-BSA. Intracellular uptake of FITC-BSA was specifically enhanced in SKOV3 cells (folate receptor over-expressing cell line) through folate receptor-mediated endocytosis compared with A549 cells (folate receptor deficient cell line). Folate-PEG-g-TMC shows promise for intracellular transport of negatively charged therapeutic proteins into folate receptor over-expressing tumor cells.
Agid:
282645