Jump to Main Content
Characterization and classification of matrix effects in biological samples analyses
- Marchi, Ivano, Viette, Véronique, Badoud, Flavia, Fathi, Marc, Saugy, Martial, Rudaz, Serge, Veuthey, Jean-Luc
- Journal of chromatography 2010 v.1217 no.25 pp. 4071-4078
- electrospray ionization mass spectrometry, liquid chromatography, solid phase extraction, urine
- An exhaustive classification of matrix effects occurring when a sample preparation is performed prior to liquid-chromatography coupled to mass spectrometry (LC-MS) analyses was proposed. A total of eight different situations were identified allowing the recognition of the matrix effect typology via the calculation of four recovery values. A set of 198 compounds was used to evaluate matrix effects after solid phase extraction (SPE) from plasma or urine samples prior to LC-ESI-MS analysis. Matrix effect identification was achieved for all compounds and classified through an organization chart. Only 17% of the tested compounds did not present significant matrix effects.