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Genotyping of Aeromonas hydrophila by Box elements

Author:
Singh, Vijai, Chaudhary, Dharmendra Kumar, Mani, Indra, Somvanshi, Pallavi, Rathore, Gaurav, Sood, Neeraj
Source:
Microbiology 2010 v.79 no.3 pp. 370-373
ISSN:
0026-2617
Subject:
Aeromonas hydrophila, DNA fingerprinting, genome, genotype, genotyping, polymerase chain reaction
Abstract:
PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500-3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers.
Agid:
285118