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Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery
- Fujita, Hideaki, Shimizu, Kazunori, Morioka, Yuki, Nagamori, Eiji
- Journal of bioscience and bioengineering 2010 v.110 no.3 pp. 359-362
- fluorescence microscopy, glucose, oxygen, perfluorocarbons
- We have studied the effect of enhanced oxygen delivery by perfluorocarbons on the differentiation of C2C12 cells. The extent of differentiation was assessed by means of phase contrast/fluorescence microscopy, active tension measurement and the glucose consumption/lactate production rates. We found that enhanced oxygen delivery is suitable for full differentiation of C2C12 cells.