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Isolation of a polysaccharide with anticancer activity from Auricularia polytricha using high-speed countercurrent chromatography with an aqueous two-phase system

Song, Guanglei, Du, Qizhen
Journal of chromatography 2010 v.1217 no.38 pp. 5930-5934
Auricularia polytricha, acetylation, anticarcinogenic activity, countercurrent chromatography, growth retardation, hydrolysis, methylation, mice, molecular weight, nuclear magnetic resonance spectroscopy, oxidation, polysaccharides, sarcoma
Polysaccharides from a crude extract of Auricularia polytricha were separated by high-speed countercurrent chromatography (HSCCC). The separation was performed with an aqueous two-phase system of PEG1000-K₂HPO₄-KH₂PO₄-H₂O (0.5:1.25:1.25:7.0, w/w). The crude sample (2.0g) was successfully separated into three polysaccharide components of AAPS-1 (192mg), AAPS-2 (137mg), and AAPS-3 (98mg) with molecular weights of 162, 259, and 483kDa, respectively. These compounds were tested for growth inhibition of transplanted S180 sarcoma in mice. AAPS-2 had an inhibition rate of 40.4%. The structure of AAPS-2 was elucidated from partial hydrolysis, periodate oxidation, acetylation, methylation analysis, and NMR spectroscopy (¹H, ¹³C). These results showed AAPS-2 is a polysaccharide with a backbone of (1→3)-linked-β-d-glucopyranosyl and (1→3, 6)-linked-β-d-glucopyranosyl residues in a 2:1 ratio, and has one terminal (1→)-β-d-glucopyranosyl at the O-6 position of (1→3, 6)-linked-β-d-glucopyranosyl of the main chain.