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Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: Experimental and modeling studies

Author:
Goh, Fernie, Gross, Jeffrey D., Simpson, Nicholas E., Sambanis, Athanassios
Source:
Journal of biotechnology 2010 v.150 no.2 pp. 232-239
ISSN:
0168-1656
Subject:
anaerobic conditions, biotechnology, calcium alginate, emulsions, encapsulation, insulin secretion, mice, models, oxygen, perfluorocarbons, solubility
Abstract:
Due to the high solubility of oxygen in perfluorocarbons (PFCs), these compounds have been explored for improved cell and tissue oxygenation. The goal of this study is to investigate the effects of a PFC emulsion on cellular growth and function in a tissue engineered construct. A perfluorotributylamine (PFTBA) emulsion was co-encapsulated at 10vol% with mouse βTC-tet insulinoma cells in calcium alginate beads and cultured under normoxic and severely hypoxic conditions. The number of metabolically active cells and the induced insulin secretion rate were measured over time for up to 16 days. Results showed no significant effect of PFTBA relative to the PFTBA-free control. The alginate-PFC-cell system was also modeled mathematically, and simulations tracked the number of viable cells over time under the same conditions used experimentally. Simulations revealed only a small, likely experimentally undetectable difference in cell density between the PFC-containing and PFC-free control beads. It is concluded that PFTBA up to 10vol% has no significant effect on the growth and function of encapsulated βTC-tet cells under normoxic and hypoxic conditions.
Agid:
287564