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Comparison and evaluation of methods for detection of tomato infectious chlorosis virus (TICV) tomato chlorosis virus (TOCV)

Author:
Suhard, P. C., Gentit, P., Poliakoff, F.
Source:
Acta horticulturae 2015 no.1069 pp. 119-126
ISSN:
0567-7572
Subject:
Tomato chlorosis virus, Tomato infectious chlorosis virus, analytical specificity, diagnostic sensitivity, diagnostic specificity, microbial detection, reverse transcriptase polymerase chain reaction, viruses
Abstract:
To review the French official detection method for TICV and ToCV, three detection methods were assessed: one RT-PCR for each virus and a Triplex real-time RT-PCR. The real-time RT-PCR is designed to detect both viruses associated with an internal control as recommended in the AFNOR standard XPV03-043. For each method, using a range of target and non-target samples, we evaluated four different criteria: analytical specificity, diagnostic sensitivity, diagnostic specificity and repeatability according to the EPPO standard OEPP PM7/98, PM7/76. Under our conditions, the four criteria were 100% for the real-time RT-PCR and for the conventional RT-PCR for the detection of the TICV whereas for TOCV the analytical specificity and diagnostic sensitivity were 95.52 and 82.46%, respectively, and 100% for each criteria for the conventional RT-PCR. This evaluation showed that the real-time RT-PCR is a sensitive, specific and robust assay that can be used to identify both viruses with good accuracy. Extensive testing including a ring trial may be set up to identify possible bottlenecks before implementation and is proposed as a forward step of our evaluation.
Agid:
2878430