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Comparison and evaluation of methods for detection of tomato infectious chlorosis virus (TICV) tomato chlorosis virus (TOCV)

Suhard, P. C., Gentit, P., Poliakoff, F.
Acta horticulturae 2015 no.1069 pp. 119-126
Tomato chlorosis virus, Tomato infectious chlorosis virus, analytical specificity, diagnostic sensitivity, diagnostic specificity, microbial detection, reverse transcriptase polymerase chain reaction, viruses
To review the French official detection method for TICV and ToCV, three detection methods were assessed: one RT-PCR for each virus and a Triplex real-time RT-PCR. The real-time RT-PCR is designed to detect both viruses associated with an internal control as recommended in the AFNOR standard XPV03-043. For each method, using a range of target and non-target samples, we evaluated four different criteria: analytical specificity, diagnostic sensitivity, diagnostic specificity and repeatability according to the EPPO standard OEPP PM7/98, PM7/76. Under our conditions, the four criteria were 100% for the real-time RT-PCR and for the conventional RT-PCR for the detection of the TICV whereas for TOCV the analytical specificity and diagnostic sensitivity were 95.52 and 82.46%, respectively, and 100% for each criteria for the conventional RT-PCR. This evaluation showed that the real-time RT-PCR is a sensitive, specific and robust assay that can be used to identify both viruses with good accuracy. Extensive testing including a ring trial may be set up to identify possible bottlenecks before implementation and is proposed as a forward step of our evaluation.